3hbiomedical   3H Biomedical AB

瑞典三生細(xì)胞生物科技有限公司可提供超過130種從正常或疾病組織中分離的原代細(xì)胞和其相關(guān)的RNA以及cDNA等產(chǎn)品。細(xì)胞類型包括干細(xì)胞，單核細(xì)胞，T細(xì)胞，B細(xì)胞，自然殺傷細(xì)胞，樹突細(xì)胞，肝細(xì)胞等。

細(xì)胞系（cell line, 主要來源于ATCC）是發(fā)生突變，轉(zhuǎn)化或具有永生性的細(xì)胞。與細(xì)胞系不同，原代細(xì)胞保留了原有細(xì)胞的特性，因此能夠更好的模擬反映出在活體內(nèi)細(xì)胞的形態(tài)，新陳代謝以及生長特性等。相比于動(dòng)物實(shí)驗(yàn)，原代細(xì)胞實(shí)驗(yàn)更為仁道，快捷，靈敏，準(zhǔn)確，不易受外界條件干擾?；谠?xì)胞的以上特性，使其成為模擬體內(nèi)環(huán)境的體外研究中為理想的研究對象。

細(xì)胞轉(zhuǎn)染

在這里你可以找到  轉(zhuǎn)染試劑盒，用于內(nèi)皮細(xì)胞，成纖維細(xì)胞，上皮細(xì)胞，間充質(zhì)干細(xì)胞，星形膠質(zhì)細(xì)胞，平滑肌細(xì)胞和角質(zhì)形成細(xì)胞的轉(zhuǎn)染。

將外源DNA遞送到真核細(xì)胞中是研究生物機(jī)制的常見的分子生物學(xué)技術(shù)之一。然而，與轉(zhuǎn)化細(xì)胞系不同，原代細(xì)胞的有效轉(zhuǎn)染可能是個(gè)問題。EndoFectagen是一種基于陽離子聚合物的轉(zhuǎn)染系統(tǒng)，專門設(shè)計(jì)和優(yōu)化用于有效轉(zhuǎn)染原代內(nèi)皮細(xì)胞。代替正常的兩天轉(zhuǎn)染，可以進(jìn)行優(yōu)化的一天轉(zhuǎn)染程序以節(jié)省時(shí)間和高度可重復(fù)的轉(zhuǎn)染。

EndoFectagen（EndoF）

細(xì)胞轉(zhuǎn)染

SC0903

FibroFectagen（FibroF）

細(xì)胞轉(zhuǎn)染

SC0913

EpiFectagen（EpiF）

細(xì)胞轉(zhuǎn)染

SC0923

MesenFectagen

細(xì)胞轉(zhuǎn)染

SC0933

AstroFectagen（AstroF）

細(xì)胞轉(zhuǎn)染

SC0943

SMCFectagen（SMCF）

細(xì)胞轉(zhuǎn)染

SC0953

Kerafectagen（KeraF）

細(xì)胞轉(zhuǎn)染

SC0963

EpiFectagen II（EpiF）

細(xì)胞轉(zhuǎn)染

SC0973

EndoFectagen（EndoF）

細(xì)胞轉(zhuǎn)染

EndoFectagen是一種基于陽離子聚合物的轉(zhuǎn)染系統(tǒng)，專門設(shè)計(jì)和優(yōu)化用于有效轉(zhuǎn)染原代內(nèi)皮細(xì)胞。EndoFectagen的轉(zhuǎn)染可以在抗生素和血清的存在下進(jìn)行。代替正常的兩天轉(zhuǎn)染，可以進(jìn)行優(yōu)化的一天轉(zhuǎn)染程序以節(jié)省時(shí)間和高度可重復(fù)的轉(zhuǎn)染。在96孔板中，1ml EndoFectagen試劑足以在每孔中進(jìn)行多達(dá)250次轉(zhuǎn)染。（SC0903）（SC0903）

描述

儲(chǔ)存/處理：收到后，將-20分鐘的EndoFectagen試劑A等分并儲(chǔ)存，避免反復(fù)凍融。解凍后，將EndoFectagen試劑A儲(chǔ)存在4°C并在一個(gè)月內(nèi)使用。EndoFectagen試劑B可保持在4°C。

質(zhì)量控制：每批EndoFectagen都通過用Promega®ρSV-bata-Galactosidase對照載體轉(zhuǎn)染人臍帶血管內(nèi)皮細(xì)胞（HUVEC，目錄號(hào)SC8000）進(jìn)行性能測試。轉(zhuǎn)染后24小時(shí)通過X-gal染色測定基因表達(dá)。通常，可以實(shí)現(xiàn)30-60％的轉(zhuǎn)染效率。

Human Primary Cells/人原代細(xì)胞

1. Peripheral Blood Cell System 外周血細(xì)胞系統(tǒng)
1.1 Human Peripheral Blood Mononuclear Cells/人外周血單核細(xì)胞
1.2 Human Peripheral Blood Pan-T Cells/人外周血Pan-T 細(xì)胞
1.3 Human Peripheral Blood CD4 T Cells/人外周血CD4 T 細(xì)胞
1.4 Human Peripheral Blood CD8 T Cells/人外周血CD8 T 細(xì)胞
1.5 Human Peripheral Blood B Lymphocytes/人外周血B 淋巴細(xì)胞
1.6 Human Peripheral Blood Natural Killer Cells/人外周血自然殺傷細(xì)胞
1.7 Human Peripheral Blood CD14 Monocytes/人外周血CD14 單核細(xì)胞
1.8 Human Monocyte-derived Macrophages/人單核細(xì)胞-衍生巨噬細(xì)胞
1.9 Human Peripheral Blood CD34 Stem Cells/人外周血CD34 干細(xì)胞
1.10 Human Peripheral Blood Monocyte-derived Immature Dendritic Cells/人外周血單核細(xì)胞-衍生未成熟樹突細(xì)胞
1.11 Human Peripheral Blood Plasmacytoid Dendritic Cells/人外周血漿樣樹突細(xì)胞
1.12 Human Peripheral Blood Myeloid Dendritic Cells/人外周血髓樣樹突細(xì)胞
1.13 Human Peripheral Blood Endothelial Progenitor Cells /人外周血內(nèi)皮祖細(xì)胞
1.14 Human Peripheral Blood Eosinophils/人外周血嗜酸性粒細(xì)胞
1.15 Human Peripheral Blood Basophils/人外周血嗜堿性粒細(xì)胞
1.16 Human Peripheral Blood Neutrophils/人外周血嗜中性白細(xì)胞
1.17 Human Peripheral Blood Platelets/人外周血血小板

2. Umbilical Cord Blood Cell System /臍帶血細(xì)胞系統(tǒng)
2.1 Human Umbilical Cord Blood Mononuclear Cells/人臍帶血單核細(xì)胞
2.2 Human Umbilical Cord Blood Pan-T Cells/人臍帶血Pan-T 細(xì)胞
2.3 Human Umbilical Cord Blood CD4 T Cells/人臍帶血CD4 T 細(xì)胞
2.4 Human Umbilical Cord Blood CD8 T Cells/人臍帶血CD8 T 細(xì)胞
2.5 Human Umbilical Cord Blood B Lymphocytes/人臍帶血B 淋巴細(xì)胞
2.6 Human Umbilical Cord Blood Natural Killer Cells/人臍帶血自然殺傷細(xì)胞
2.7 Human Umbilical Cord Blood CD14 Monocytes/人臍帶血CD14 單核細(xì)胞
2.8 Human Umbilical Cord Blood CD34 Stem Cells/人臍帶血CD34 干細(xì)胞
2.9 Human Umbilical Cord Blood CD133 Stem Cells/人臍帶血CD133 干細(xì)胞
2.10 Human Umbilical Cord Blood Mesenchymal Stem Cells/人臍帶血間充質(zhì)干細(xì)胞
2.11 Human Umbilical Cord Blood Monocyte-derived Immature Dendritic Cells/人臍帶血單核細(xì)胞-衍生未成熟樹突細(xì)胞
2.12 Human Umbilical Cord Blood Plasmacytoid Dendritic Cells/人臍帶血漿樣樹突細(xì)胞
2.13 Human Umbilical Cord Blood Myeloid Dendritic Cells/人臍帶血髓樣樹突細(xì)胞
2.14 Human Umbilical Cord Blood Endothelial Progenitor Cells/人臍帶血內(nèi)皮祖細(xì)胞
2.15 Human Umbilical Cord Blood Eosinophils/人臍帶血嗜酸性粒細(xì)胞
2.16 Human Umbilical Cord Blood Basophils/人臍帶血嗜堿性粒細(xì)胞
2.17 Human Umbilical Cord Blood Neutrophils/人臍帶血嗜中性白細(xì)胞

人脈絡(luò)叢內(nèi)皮細(xì)胞

3. Hepatic Cell System/肝細(xì)胞系統(tǒng)
3.1 Fresh Human Hepatocytes /新鮮人肝細(xì)胞
3.2 Frozen Human Hepatocytes/凍存人肝細(xì)胞
3.3 Related Human Hepatic Cell Products/人肝細(xì)胞相關(guān)產(chǎn)品

Human Cell Lines/人類細(xì)胞株

4. Human Hepatic Cell Lines/人肝細(xì)胞株
4.1 HepaRG
4.2 HepaRG 細(xì)胞系

Animal Primary Cells/動(dòng)物原代細(xì)胞

5. Mouse Cell Systems/小鼠細(xì)胞系統(tǒng)
5.1 Mouse Splenocytes – available from several mouse strains/小鼠脾細(xì)胞-多種小鼠類型可供選擇
5.2 Mouse Peripheral Blood Mononuclear Cells – available from several mouse strains/小鼠外周血單核細(xì)胞-多種小鼠類型可供選擇
5.3 Mouse CD4 T Cells – available from several mouse strains /小鼠CD4 T 細(xì)胞-多種小鼠類型可供選擇
5.4 Mouse CD8 T Cells – available from several mouse strains/小鼠CD8 T 細(xì)胞-多種小鼠類型可供選擇
5.5 Mouse B Cells – available from several mouse strains/小鼠B 細(xì)胞-多種小鼠類型可供選擇
5.6 Mouse Bone Marrow Cells – available from several mouse strains /小鼠骨髓細(xì)胞-多種小鼠類型可供選擇
5.7 Mouse Neutrophils – available from several mouse strains/小鼠中性粒細(xì)胞-多種小鼠類型可供選擇
5.8 Fresh Mouse Hepatocytes/新鮮小鼠肝細(xì)胞
5.9 Frozen Mouse Hepatocytes/凍存小鼠肝細(xì)胞
5.10 Related Mouse Hepatic Cell Products/小鼠肝細(xì)胞相關(guān)產(chǎn)品

6. Rat Cell Systems/大鼠細(xì)胞系統(tǒng)
6.1 Rat Splenocytes – available from several rat strains/大鼠脾細(xì)胞-多種大鼠類型可供選擇
6.2 Rat Peripheral Blood Mononuclear Cells – available from several rat strains/大鼠外周血單核細(xì)胞-多種大鼠類型可供選擇
6.3 Rat CD4 T Cells – available from several rat strains/大鼠CD4 T 細(xì)胞-多種大鼠類型可供選擇
6.4 Rat CD8 T Cells – available from several rat strains/大鼠CD8 T 細(xì)胞-多種大鼠類型可供選擇
6.5 Rat B Cells – available from several rat strains/大鼠B 細(xì)胞-多種大鼠類型可供選擇
6.6 Rat Bone Marrow Cells – available from several rat strains/大鼠骨髓細(xì)胞-多種大鼠類型可供選擇
6.7 Rat Neutrophils – available from several rat strains/大鼠中性粒細(xì)胞-多種大鼠類型可供選擇
6.8 Fresh Sprague Dawley Rat Hepatocytes/新鮮Sprague-Dawley 大鼠肝細(xì)胞
6.9 Frozen Sprague Dawley Rat Hepatocytes/凍存Sprague-Dawley 大鼠肝細(xì)胞
6.10 Related Sprague Dawley Rat Hepatic Cell Products/Sprague-Dawley 大鼠肝細(xì)胞相關(guān)產(chǎn)品
6.11 Fresh Wistar Rat Hepatocytes/新鮮Wistar 大鼠肝細(xì)胞
6.12 Frozen Wistar Rat Hepatocytes/凍存Wistar 大鼠肝細(xì)胞
6.13 Related Wistar Rat Hepatic Cell Products/Wistar 大鼠肝細(xì)胞相關(guān)產(chǎn)品

7. Dog Cell Systems/狗細(xì)胞系統(tǒng)
7.1 Fresh Beagle Dog Hepatocytes/新鮮比格犬肝細(xì)胞
7.2 Frozen Beagle Dog Hepatocytes/凍存比格犬肝細(xì)胞
7.3 Related Beagle Dog Hepatic Cell Products/比格犬肝細(xì)胞相關(guān)產(chǎn)品
7.4 Dog Peripheral Blood Mononuclear Cells/狗外周血單核細(xì)胞

8. Monkey Cell Systems/猴細(xì)胞系統(tǒng)
8.1 Fresh Cynomolgus Monkey Hepatocytes/新鮮食蟹猴肝細(xì)胞
8.2 Frozen Cynomolgus Monkey Hepatocytes/凍存食蟹猴肝細(xì)胞
8.3 Related Cynomolgus Monkey Hepatic Cell Products/食蟹猴肝細(xì)胞相關(guān)產(chǎn)品
8.4 Frozen Marmoset Monkey Hepatocytes/凍存絨猴肝細(xì)胞
8.5 Related Marmoset Monkey Hepatic Cell Products /絨猴肝細(xì)胞相關(guān)產(chǎn)品

9. Rabbit Cell Systems/兔細(xì)胞系統(tǒng)
9.1 Fresh Rabbit Hepatocytes/新鮮兔肝細(xì)胞
9.2 Frozen Rabbit Hepatocytes/凍存兔肝細(xì)胞
9.3 Related Rabbit Hepatic Cell Products/兔肝細(xì)胞相關(guān)產(chǎn)品

10. Pig Cell Systems/豬細(xì)胞系統(tǒng)
10.1 Fresh Minipig Hepatocytes/新鮮小型豬肝細(xì)胞
10.2 Frozen Minipig Hepatocytes/凍存小型豬肝細(xì)胞
10.3 Related Minipig Hepatic Cell Products/小型豬肝細(xì)胞相關(guān)產(chǎn)品

11. Guinea Pig Cell Systems/豚鼠細(xì)胞系統(tǒng)
11.1 Frozen Guinea Pig Hepatocytes/凍存豚鼠肝細(xì)胞
11.2 Related Guinea Pig Hepatic Cell Products/豚鼠肝細(xì)胞相關(guān)產(chǎn)品

12. Hamster Cell Systems/倉鼠細(xì)胞系統(tǒng)
12.1 Frozen Hamster Hepatocytes/凍存?zhèn)}鼠肝細(xì)胞
12.2 Related Hamster Hepatic Cell Products/倉鼠肝細(xì)胞相關(guān)產(chǎn)品

Culture Medium and Supplements/細(xì)胞培養(yǎng)基及添加物

13.1 Cell Culture Medium (see also under each cell type category/細(xì)胞培養(yǎng)基
13.2 Cell Thawing Media/細(xì)胞解凍培養(yǎng)基
13.3 Cell Freezing Media/細(xì)胞凍存培養(yǎng)基
13.4 Cell Detach Media/細(xì)胞脫附培養(yǎng)基
13.5 Fetal Bovine Serum/胎牛血清
13.6 Supplements and Reagents/添加物及試劑
13.7 Balanced Salt Solutions and Water/平衡鹽溶液及水

Cell Culture Model Systems/細(xì)胞培養(yǎng)模式系統(tǒng)

14.1 Human LiverbeadsTM/人肝細(xì)胞粒
14.2 Mouse LiverbeadsTM/小鼠肝細(xì)胞粒
14.3 Rat LiverbeadsTM/大鼠肝細(xì)胞粒
14.4 Dog LiverbeadsTM/狗肝細(xì)胞粒
14.5 Monkey LiverbeadsTM/猴肝細(xì)胞粒
14.6 Pig LiverbeadsTM/豬肝細(xì)胞粒
14.7 Human Hepatocyte Sandwich Culture/人肝細(xì)胞夾層膠原三明治培養(yǎng)基
14.8 Rat Hepatocyte Sandwich Culture/大鼠肝細(xì)胞夾層膠原三明治培養(yǎng)基

Tissue Engineering Systems/組織工程系統(tǒng)

15.1 Human Liver Slices/人肝切片
15.2 Rat Liver Slices/大鼠肝切片

Sera/血清

16.1 Normal Human Serum/正常人血清
16.2 Normal Human AB Serum/正常人AB 血清

Plasma/血漿

17.1 Normal Human Plasma/正常人血漿
17.2 Human Nickel Plasma/人鎳過敏血漿

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